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Applications of BBa_K5299001

The pTNS helper plasmid carries the genes encoding components of the Tn7 transposase (TnSABC+D operon). Tn7 transposase recognizes Tn7L and Tn7R. The production of the transposase leads to the integration of the DNA region between the Tn7L and Tn7R regions from the plasmid pTN7-M into the bacterial chromosome at the attTN7 site. The attTn7 site is 25 bp downstream of the glmS genes. [1] The plasmid’s origin of replication is the R6K ori that requires the presence of the PIR protein. [2] The antibiotic marker of the plasmid is Αmpicillin.

[1] Choi, K.-H., & Schweizer, H. P. (2006). mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. In Nature Protocols (Vol. 1, Issue 1, pp. 153–161). Springer Science and Business Media LLC. https://doi.org/10.1038/nprot.2006.24

[2] Kaniga, K., Delor, I., & Cornelis, G. R. (1991). A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. In Gene (Vol. 109, Issue 1, pp. 137–141). Elsevier BV. https://doi.org/10.1016/0378-1119(91)90599-7

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